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cell lines raw264 7 american type culture collection tib  (ATCC)


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    ATCC cell lines raw264 7 american type culture collection tib
    Cell Lines Raw264 7 American Type Culture Collection Tib, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 24592 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+lines+raw264+7+american+type+culture+collection+tib/RAW+264%2E7/pm41926285-589-124-127
    Average 99 stars, based on 24592 article reviews
    cell lines raw264 7 american type culture collection tib - by Bioz Stars, 2026-10
    99/100 stars

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    Recombinant:

    Article Title: The rebalancing of the immune system at the maternal-fetal interface ameliorates autism-like behavior in adult offspring.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human TGF-beta 1 Recombinant Protein PeproTech Cat# 100-21C-50UG Phosphatase inhibitor Beyotime Cat# P1082 Protease inhibitor cocktail Beyotime Cat# P1046 Recombinant Murine M-CSF PeproTech Cat# 315-02 chlorophosphate liposomes YEASEN Cat# 40337ES08 EDTA Antigen Retrieval Solution Beyotime Cat# P0085 DAPI Fluoromount Southern Biotech Cat# 0100-20 Critical commercial assays TRIzol reagent Invitrogen Cat# 15596018 HiScript II Q Select RT SuperMix Vazyme Cat# R222-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat# Q511-03 CellTraceTM CFSE Cell Proliferation Kit ThermoFisher Scientific Cat# C34554 Deposited data Single-cell RNAseq This paper PRJNA1152012 RNA-seq This paper PRJNA1055261 Experimental models: Cell lines RAW264.7 American Type Culture Collection TIB-71 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory 000664 Mouse: Foxp3GFP Prof. Chuan Su, Nanjing Medical University N/A Mouse: Foxp3DTR Prof. Xiaoming Wang, Nanjing Medical University N/A Oligonucleotides See Table S1 Software and algorithms Fiji/ImageJ ImageJ https://imagej.net/software/fiji/ FlowJo FlowJo https://www.flowjo.com/solutions/flowjo Prism Graph Pad https://www.graphpad.com/ Image-ProPlus software 6.0 Media Cybernetics https://image-pro-plus.software. informer.com/6.0/ SuperMaze software Shanghai XinRuan Information Technology N/A ..

    Protease Inhibitor:

    Article Title: The rebalancing of the immune system at the maternal-fetal interface ameliorates autism-like behavior in adult offspring.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human TGF-beta 1 Recombinant Protein PeproTech Cat# 100-21C-50UG Phosphatase inhibitor Beyotime Cat# P1082 Protease inhibitor cocktail Beyotime Cat# P1046 Recombinant Murine M-CSF PeproTech Cat# 315-02 chlorophosphate liposomes YEASEN Cat# 40337ES08 EDTA Antigen Retrieval Solution Beyotime Cat# P0085 DAPI Fluoromount Southern Biotech Cat# 0100-20 Critical commercial assays TRIzol reagent Invitrogen Cat# 15596018 HiScript II Q Select RT SuperMix Vazyme Cat# R222-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat# Q511-03 CellTraceTM CFSE Cell Proliferation Kit ThermoFisher Scientific Cat# C34554 Deposited data Single-cell RNAseq This paper PRJNA1152012 RNA-seq This paper PRJNA1055261 Experimental models: Cell lines RAW264.7 American Type Culture Collection TIB-71 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory 000664 Mouse: Foxp3GFP Prof. Chuan Su, Nanjing Medical University N/A Mouse: Foxp3DTR Prof. Xiaoming Wang, Nanjing Medical University N/A Oligonucleotides See Table S1 Software and algorithms Fiji/ImageJ ImageJ https://imagej.net/software/fiji/ FlowJo FlowJo https://www.flowjo.com/solutions/flowjo Prism Graph Pad https://www.graphpad.com/ Image-ProPlus software 6.0 Media Cybernetics https://image-pro-plus.software. informer.com/6.0/ SuperMaze software Shanghai XinRuan Information Technology N/A ..

    Liposomes:

    Article Title: The rebalancing of the immune system at the maternal-fetal interface ameliorates autism-like behavior in adult offspring.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human TGF-beta 1 Recombinant Protein PeproTech Cat# 100-21C-50UG Phosphatase inhibitor Beyotime Cat# P1082 Protease inhibitor cocktail Beyotime Cat# P1046 Recombinant Murine M-CSF PeproTech Cat# 315-02 chlorophosphate liposomes YEASEN Cat# 40337ES08 EDTA Antigen Retrieval Solution Beyotime Cat# P0085 DAPI Fluoromount Southern Biotech Cat# 0100-20 Critical commercial assays TRIzol reagent Invitrogen Cat# 15596018 HiScript II Q Select RT SuperMix Vazyme Cat# R222-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat# Q511-03 CellTraceTM CFSE Cell Proliferation Kit ThermoFisher Scientific Cat# C34554 Deposited data Single-cell RNAseq This paper PRJNA1152012 RNA-seq This paper PRJNA1055261 Experimental models: Cell lines RAW264.7 American Type Culture Collection TIB-71 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory 000664 Mouse: Foxp3GFP Prof. Chuan Su, Nanjing Medical University N/A Mouse: Foxp3DTR Prof. Xiaoming Wang, Nanjing Medical University N/A Oligonucleotides See Table S1 Software and algorithms Fiji/ImageJ ImageJ https://imagej.net/software/fiji/ FlowJo FlowJo https://www.flowjo.com/solutions/flowjo Prism Graph Pad https://www.graphpad.com/ Image-ProPlus software 6.0 Media Cybernetics https://image-pro-plus.software. informer.com/6.0/ SuperMaze software Shanghai XinRuan Information Technology N/A ..

    Real-time Polymerase Chain Reaction:

    Article Title: The rebalancing of the immune system at the maternal-fetal interface ameliorates autism-like behavior in adult offspring.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human TGF-beta 1 Recombinant Protein PeproTech Cat# 100-21C-50UG Phosphatase inhibitor Beyotime Cat# P1082 Protease inhibitor cocktail Beyotime Cat# P1046 Recombinant Murine M-CSF PeproTech Cat# 315-02 chlorophosphate liposomes YEASEN Cat# 40337ES08 EDTA Antigen Retrieval Solution Beyotime Cat# P0085 DAPI Fluoromount Southern Biotech Cat# 0100-20 Critical commercial assays TRIzol reagent Invitrogen Cat# 15596018 HiScript II Q Select RT SuperMix Vazyme Cat# R222-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat# Q511-03 CellTraceTM CFSE Cell Proliferation Kit ThermoFisher Scientific Cat# C34554 Deposited data Single-cell RNAseq This paper PRJNA1152012 RNA-seq This paper PRJNA1055261 Experimental models: Cell lines RAW264.7 American Type Culture Collection TIB-71 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory 000664 Mouse: Foxp3GFP Prof. Chuan Su, Nanjing Medical University N/A Mouse: Foxp3DTR Prof. Xiaoming Wang, Nanjing Medical University N/A Oligonucleotides See Table S1 Software and algorithms Fiji/ImageJ ImageJ https://imagej.net/software/fiji/ FlowJo FlowJo https://www.flowjo.com/solutions/flowjo Prism Graph Pad https://www.graphpad.com/ Image-ProPlus software 6.0 Media Cybernetics https://image-pro-plus.software. informer.com/6.0/ SuperMaze software Shanghai XinRuan Information Technology N/A ..

    Article Title: Maternal immune activation perturbs intestinal niche through microbial glycerophospholipids and drives offspring behavioral abnormalities.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER DMEM WISENT Cat# 319-205-CL Tween 20 Sigma-Aldrich Cat# P1379 Triton X-100 Sigma-Aldrich Cat# 93443 bovine serum albumin Sigma-Aldrich Cat# V900933 SDS-PAGE Sample Loading Buffer Beyotime Biotechnology Cat# P0015 RIPA Complete Lysis Buffer Beyotime Biotechnology Cat# P0038 0.25% Trypsin-EDTA (1×) Gibco Cat# 25200072 180 kDa Plus Prestained Protein Marker Vazyme Cat# MP201 DMSO Invitrogen Cat# D12345 InStabTM Protease Cocktail YEASEN Cat# 20124ES03 DAPI Solarbio Cat# 28718-90-3 Critical commercial assays Enzyme-linked immunosorbent assay Elabscience Cat# E-EL-M0044 TRIzol Invitrogen Cat# 15596018CN HiScript II Q Select RT SuperMix Vazyme Cat# R222-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat# Q511-03 Deposited data 16S rRNA-seq This paper PRJNA1159518, https://www.ncbi.nlm.nih. gov/sra/?term=PRJNA1159518 Proteomics This paper PXD075246, https://proteomecentral. proteomexchange.org RNA-seq This paper PRJNA1345435, https://www.ncbi.nlm. nih.gov/sra/?term=PRJNA1345435 Experimental models: Cell lines RAW264.7 American Type Culture Collection TIB-71 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory IMSR_JAX:000664 Mouse: IL-6 Knockout Xinghua Gao, China Pharmaceutical University N/A Oligonucleotides See Table S1 N/A N/A Software and algorithms GraphPad Prism Graph Pad https://www.graphpad.com/ FlowJo FlowJo https://www.flowjo.com/solutions/flowjo Image-ProPlus software 6.0 Media Cybernetics https://image-pro-plus.software. informer.com/6.0/ Adobe Illustrator Adobe https://www.adobe.com SuperMaze software Shanghai XinRuan Information Technology https://www.shxinruan.com/ Other T. gondii soluble tachyzoite antigen (STAg) This paper N/A 18 Cell Reports 45, 117211, April 28, 2026 .. Offspring from pregnant mice intraperitoneally injected with parasitic mimetics (soluble tachyzoite antigen, STAg) or synthetic double-stranded RNA (dsRNA) Poly(I:C) (Sigma-Aldrich, St. Louis, USA), a viral infection mimic, exhibited behavioral abnormalities, including social deficits, impaired communication, and repetitive behaviors.8,14 For mating, male and female mice were housed together overnight, and females were examined for the presence of seminal plugs the following morning.

    SYBR Green Assay:

    Article Title: The rebalancing of the immune system at the maternal-fetal interface ameliorates autism-like behavior in adult offspring.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human TGF-beta 1 Recombinant Protein PeproTech Cat# 100-21C-50UG Phosphatase inhibitor Beyotime Cat# P1082 Protease inhibitor cocktail Beyotime Cat# P1046 Recombinant Murine M-CSF PeproTech Cat# 315-02 chlorophosphate liposomes YEASEN Cat# 40337ES08 EDTA Antigen Retrieval Solution Beyotime Cat# P0085 DAPI Fluoromount Southern Biotech Cat# 0100-20 Critical commercial assays TRIzol reagent Invitrogen Cat# 15596018 HiScript II Q Select RT SuperMix Vazyme Cat# R222-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat# Q511-03 CellTraceTM CFSE Cell Proliferation Kit ThermoFisher Scientific Cat# C34554 Deposited data Single-cell RNAseq This paper PRJNA1152012 RNA-seq This paper PRJNA1055261 Experimental models: Cell lines RAW264.7 American Type Culture Collection TIB-71 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory 000664 Mouse: Foxp3GFP Prof. Chuan Su, Nanjing Medical University N/A Mouse: Foxp3DTR Prof. Xiaoming Wang, Nanjing Medical University N/A Oligonucleotides See Table S1 Software and algorithms Fiji/ImageJ ImageJ https://imagej.net/software/fiji/ FlowJo FlowJo https://www.flowjo.com/solutions/flowjo Prism Graph Pad https://www.graphpad.com/ Image-ProPlus software 6.0 Media Cybernetics https://image-pro-plus.software. informer.com/6.0/ SuperMaze software Shanghai XinRuan Information Technology N/A ..

    Article Title: Maternal immune activation perturbs intestinal niche through microbial glycerophospholipids and drives offspring behavioral abnormalities.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER DMEM WISENT Cat# 319-205-CL Tween 20 Sigma-Aldrich Cat# P1379 Triton X-100 Sigma-Aldrich Cat# 93443 bovine serum albumin Sigma-Aldrich Cat# V900933 SDS-PAGE Sample Loading Buffer Beyotime Biotechnology Cat# P0015 RIPA Complete Lysis Buffer Beyotime Biotechnology Cat# P0038 0.25% Trypsin-EDTA (1×) Gibco Cat# 25200072 180 kDa Plus Prestained Protein Marker Vazyme Cat# MP201 DMSO Invitrogen Cat# D12345 InStabTM Protease Cocktail YEASEN Cat# 20124ES03 DAPI Solarbio Cat# 28718-90-3 Critical commercial assays Enzyme-linked immunosorbent assay Elabscience Cat# E-EL-M0044 TRIzol Invitrogen Cat# 15596018CN HiScript II Q Select RT SuperMix Vazyme Cat# R222-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat# Q511-03 Deposited data 16S rRNA-seq This paper PRJNA1159518, https://www.ncbi.nlm.nih. gov/sra/?term=PRJNA1159518 Proteomics This paper PXD075246, https://proteomecentral. proteomexchange.org RNA-seq This paper PRJNA1345435, https://www.ncbi.nlm. nih.gov/sra/?term=PRJNA1345435 Experimental models: Cell lines RAW264.7 American Type Culture Collection TIB-71 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory IMSR_JAX:000664 Mouse: IL-6 Knockout Xinghua Gao, China Pharmaceutical University N/A Oligonucleotides See Table S1 N/A N/A Software and algorithms GraphPad Prism Graph Pad https://www.graphpad.com/ FlowJo FlowJo https://www.flowjo.com/solutions/flowjo Image-ProPlus software 6.0 Media Cybernetics https://image-pro-plus.software. informer.com/6.0/ Adobe Illustrator Adobe https://www.adobe.com SuperMaze software Shanghai XinRuan Information Technology https://www.shxinruan.com/ Other T. gondii soluble tachyzoite antigen (STAg) This paper N/A 18 Cell Reports 45, 117211, April 28, 2026 .. Offspring from pregnant mice intraperitoneally injected with parasitic mimetics (soluble tachyzoite antigen, STAg) or synthetic double-stranded RNA (dsRNA) Poly(I:C) (Sigma-Aldrich, St. Louis, USA), a viral infection mimic, exhibited behavioral abnormalities, including social deficits, impaired communication, and repetitive behaviors.8,14 For mating, male and female mice were housed together overnight, and females were examined for the presence of seminal plugs the following morning.

    Single Cell:

    Article Title: The rebalancing of the immune system at the maternal-fetal interface ameliorates autism-like behavior in adult offspring.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human TGF-beta 1 Recombinant Protein PeproTech Cat# 100-21C-50UG Phosphatase inhibitor Beyotime Cat# P1082 Protease inhibitor cocktail Beyotime Cat# P1046 Recombinant Murine M-CSF PeproTech Cat# 315-02 chlorophosphate liposomes YEASEN Cat# 40337ES08 EDTA Antigen Retrieval Solution Beyotime Cat# P0085 DAPI Fluoromount Southern Biotech Cat# 0100-20 Critical commercial assays TRIzol reagent Invitrogen Cat# 15596018 HiScript II Q Select RT SuperMix Vazyme Cat# R222-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat# Q511-03 CellTraceTM CFSE Cell Proliferation Kit ThermoFisher Scientific Cat# C34554 Deposited data Single-cell RNAseq This paper PRJNA1152012 RNA-seq This paper PRJNA1055261 Experimental models: Cell lines RAW264.7 American Type Culture Collection TIB-71 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory 000664 Mouse: Foxp3GFP Prof. Chuan Su, Nanjing Medical University N/A Mouse: Foxp3DTR Prof. Xiaoming Wang, Nanjing Medical University N/A Oligonucleotides See Table S1 Software and algorithms Fiji/ImageJ ImageJ https://imagej.net/software/fiji/ FlowJo FlowJo https://www.flowjo.com/solutions/flowjo Prism Graph Pad https://www.graphpad.com/ Image-ProPlus software 6.0 Media Cybernetics https://image-pro-plus.software. informer.com/6.0/ SuperMaze software Shanghai XinRuan Information Technology N/A ..

    RNA sequencing:

    Article Title: The rebalancing of the immune system at the maternal-fetal interface ameliorates autism-like behavior in adult offspring.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human TGF-beta 1 Recombinant Protein PeproTech Cat# 100-21C-50UG Phosphatase inhibitor Beyotime Cat# P1082 Protease inhibitor cocktail Beyotime Cat# P1046 Recombinant Murine M-CSF PeproTech Cat# 315-02 chlorophosphate liposomes YEASEN Cat# 40337ES08 EDTA Antigen Retrieval Solution Beyotime Cat# P0085 DAPI Fluoromount Southern Biotech Cat# 0100-20 Critical commercial assays TRIzol reagent Invitrogen Cat# 15596018 HiScript II Q Select RT SuperMix Vazyme Cat# R222-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat# Q511-03 CellTraceTM CFSE Cell Proliferation Kit ThermoFisher Scientific Cat# C34554 Deposited data Single-cell RNAseq This paper PRJNA1152012 RNA-seq This paper PRJNA1055261 Experimental models: Cell lines RAW264.7 American Type Culture Collection TIB-71 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory 000664 Mouse: Foxp3GFP Prof. Chuan Su, Nanjing Medical University N/A Mouse: Foxp3DTR Prof. Xiaoming Wang, Nanjing Medical University N/A Oligonucleotides See Table S1 Software and algorithms Fiji/ImageJ ImageJ https://imagej.net/software/fiji/ FlowJo FlowJo https://www.flowjo.com/solutions/flowjo Prism Graph Pad https://www.graphpad.com/ Image-ProPlus software 6.0 Media Cybernetics https://image-pro-plus.software. informer.com/6.0/ SuperMaze software Shanghai XinRuan Information Technology N/A ..

    RNA Sequencing:

    Article Title: The rebalancing of the immune system at the maternal-fetal interface ameliorates autism-like behavior in adult offspring.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human TGF-beta 1 Recombinant Protein PeproTech Cat# 100-21C-50UG Phosphatase inhibitor Beyotime Cat# P1082 Protease inhibitor cocktail Beyotime Cat# P1046 Recombinant Murine M-CSF PeproTech Cat# 315-02 chlorophosphate liposomes YEASEN Cat# 40337ES08 EDTA Antigen Retrieval Solution Beyotime Cat# P0085 DAPI Fluoromount Southern Biotech Cat# 0100-20 Critical commercial assays TRIzol reagent Invitrogen Cat# 15596018 HiScript II Q Select RT SuperMix Vazyme Cat# R222-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat# Q511-03 CellTraceTM CFSE Cell Proliferation Kit ThermoFisher Scientific Cat# C34554 Deposited data Single-cell RNAseq This paper PRJNA1152012 RNA-seq This paper PRJNA1055261 Experimental models: Cell lines RAW264.7 American Type Culture Collection TIB-71 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory 000664 Mouse: Foxp3GFP Prof. Chuan Su, Nanjing Medical University N/A Mouse: Foxp3DTR Prof. Xiaoming Wang, Nanjing Medical University N/A Oligonucleotides See Table S1 Software and algorithms Fiji/ImageJ ImageJ https://imagej.net/software/fiji/ FlowJo FlowJo https://www.flowjo.com/solutions/flowjo Prism Graph Pad https://www.graphpad.com/ Image-ProPlus software 6.0 Media Cybernetics https://image-pro-plus.software. informer.com/6.0/ SuperMaze software Shanghai XinRuan Information Technology N/A ..

    Article Title: Maternal immune activation perturbs intestinal niche through microbial glycerophospholipids and drives offspring behavioral abnormalities.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER DMEM WISENT Cat# 319-205-CL Tween 20 Sigma-Aldrich Cat# P1379 Triton X-100 Sigma-Aldrich Cat# 93443 bovine serum albumin Sigma-Aldrich Cat# V900933 SDS-PAGE Sample Loading Buffer Beyotime Biotechnology Cat# P0015 RIPA Complete Lysis Buffer Beyotime Biotechnology Cat# P0038 0.25% Trypsin-EDTA (1×) Gibco Cat# 25200072 180 kDa Plus Prestained Protein Marker Vazyme Cat# MP201 DMSO Invitrogen Cat# D12345 InStabTM Protease Cocktail YEASEN Cat# 20124ES03 DAPI Solarbio Cat# 28718-90-3 Critical commercial assays Enzyme-linked immunosorbent assay Elabscience Cat# E-EL-M0044 TRIzol Invitrogen Cat# 15596018CN HiScript II Q Select RT SuperMix Vazyme Cat# R222-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat# Q511-03 Deposited data 16S rRNA-seq This paper PRJNA1159518, https://www.ncbi.nlm.nih. gov/sra/?term=PRJNA1159518 Proteomics This paper PXD075246, https://proteomecentral. proteomexchange.org RNA-seq This paper PRJNA1345435, https://www.ncbi.nlm. nih.gov/sra/?term=PRJNA1345435 Experimental models: Cell lines RAW264.7 American Type Culture Collection TIB-71 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory IMSR_JAX:000664 Mouse: IL-6 Knockout Xinghua Gao, China Pharmaceutical University N/A Oligonucleotides See Table S1 N/A N/A Software and algorithms GraphPad Prism Graph Pad https://www.graphpad.com/ FlowJo FlowJo https://www.flowjo.com/solutions/flowjo Image-ProPlus software 6.0 Media Cybernetics https://image-pro-plus.software. informer.com/6.0/ Adobe Illustrator Adobe https://www.adobe.com SuperMaze software Shanghai XinRuan Information Technology https://www.shxinruan.com/ Other T. gondii soluble tachyzoite antigen (STAg) This paper N/A 18 Cell Reports 45, 117211, April 28, 2026 .. Offspring from pregnant mice intraperitoneally injected with parasitic mimetics (soluble tachyzoite antigen, STAg) or synthetic double-stranded RNA (dsRNA) Poly(I:C) (Sigma-Aldrich, St. Louis, USA), a viral infection mimic, exhibited behavioral abnormalities, including social deficits, impaired communication, and repetitive behaviors.8,14 For mating, male and female mice were housed together overnight, and females were examined for the presence of seminal plugs the following morning.

    Software:

    Article Title: The rebalancing of the immune system at the maternal-fetal interface ameliorates autism-like behavior in adult offspring.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Human TGF-beta 1 Recombinant Protein PeproTech Cat# 100-21C-50UG Phosphatase inhibitor Beyotime Cat# P1082 Protease inhibitor cocktail Beyotime Cat# P1046 Recombinant Murine M-CSF PeproTech Cat# 315-02 chlorophosphate liposomes YEASEN Cat# 40337ES08 EDTA Antigen Retrieval Solution Beyotime Cat# P0085 DAPI Fluoromount Southern Biotech Cat# 0100-20 Critical commercial assays TRIzol reagent Invitrogen Cat# 15596018 HiScript II Q Select RT SuperMix Vazyme Cat# R222-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat# Q511-03 CellTraceTM CFSE Cell Proliferation Kit ThermoFisher Scientific Cat# C34554 Deposited data Single-cell RNAseq This paper PRJNA1152012 RNA-seq This paper PRJNA1055261 Experimental models: Cell lines RAW264.7 American Type Culture Collection TIB-71 Experimental models: Organisms/strains Mouse: C57BL/6J Jackson Laboratory 000664 Mouse: Foxp3GFP Prof. Chuan Su, Nanjing Medical University N/A Mouse: Foxp3DTR Prof. Xiaoming Wang, Nanjing Medical University N/A Oligonucleotides See Table S1 Software and algorithms Fiji/ImageJ ImageJ https://imagej.net/software/fiji/ FlowJo FlowJo https://www.flowjo.com/solutions/flowjo Prism Graph Pad https://www.graphpad.com/ Image-ProPlus software 6.0 Media Cybernetics https://image-pro-plus.software. informer.com/6.0/ SuperMaze software Shanghai XinRuan Information Technology N/A ..

    Article Title: Maternal immune activation perturbs intestinal niche through microbial glycerophospholipids and drives offspring behavioral abnormalities.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER DMEM WISENT Cat# 319-205-CL Tween 20 Sigma-Aldrich Cat# P1379 Triton X-100 Sigma-Aldrich Cat# 93443 bovine serum albumin Sigma-Aldrich Cat# V900933 SDS-PAGE Sample Loading Buffer Beyotime Biotechnology Cat# P0015 RIPA Complete Lysis Buffer Beyotime Biotechnology Cat# P0038 0.25% Trypsin-EDTA (1×) Gibco Cat# 25200072 180 kDa Plus Prestained Protein Marker Vazyme Cat# MP201 DMSO Invitrogen Cat# D12345 InStabTM Protease Cocktail YEASEN Cat# 20124ES03 DAPI Solarbio Cat# 28718-90-3 Critical commercial assays Enzyme-linked immunosorbent assay Elabscience Cat# E-EL-M0044 TRIzol Invitrogen Cat# 15596018CN HiScript II Q Select RT SuperMix Vazyme Cat# R222-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat# Q511-03 Deposited data 16S rRNA-seq This paper PRJNA1159518, https://www.ncbi.nlm.nih. gov/sra/?term=PRJNA1159518 Proteomics This paper PXD075246, https://proteomecentral. proteomexchange.org RNA-seq This paper PRJNA1345435, https://www.ncbi.nlm. nih.gov/sra/?term=PRJNA1345435 Experimental models: Cell lines RAW264.7 American Type Culture Collection TIB-71 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory IMSR_JAX:000664 Mouse: IL-6 Knockout Xinghua Gao, China Pharmaceutical University N/A Oligonucleotides See Table S1 N/A N/A Software and algorithms GraphPad Prism Graph Pad https://www.graphpad.com/ FlowJo FlowJo https://www.flowjo.com/solutions/flowjo Image-ProPlus software 6.0 Media Cybernetics https://image-pro-plus.software. informer.com/6.0/ Adobe Illustrator Adobe https://www.adobe.com SuperMaze software Shanghai XinRuan Information Technology https://www.shxinruan.com/ Other T. gondii soluble tachyzoite antigen (STAg) This paper N/A 18 Cell Reports 45, 117211, April 28, 2026 .. Offspring from pregnant mice intraperitoneally injected with parasitic mimetics (soluble tachyzoite antigen, STAg) or synthetic double-stranded RNA (dsRNA) Poly(I:C) (Sigma-Aldrich, St. Louis, USA), a viral infection mimic, exhibited behavioral abnormalities, including social deficits, impaired communication, and repetitive behaviors.8,14 For mating, male and female mice were housed together overnight, and females were examined for the presence of seminal plugs the following morning.

    SDS Page:

    Article Title: Maternal immune activation perturbs intestinal niche through microbial glycerophospholipids and drives offspring behavioral abnormalities.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER DMEM WISENT Cat# 319-205-CL Tween 20 Sigma-Aldrich Cat# P1379 Triton X-100 Sigma-Aldrich Cat# 93443 bovine serum albumin Sigma-Aldrich Cat# V900933 SDS-PAGE Sample Loading Buffer Beyotime Biotechnology Cat# P0015 RIPA Complete Lysis Buffer Beyotime Biotechnology Cat# P0038 0.25% Trypsin-EDTA (1×) Gibco Cat# 25200072 180 kDa Plus Prestained Protein Marker Vazyme Cat# MP201 DMSO Invitrogen Cat# D12345 InStabTM Protease Cocktail YEASEN Cat# 20124ES03 DAPI Solarbio Cat# 28718-90-3 Critical commercial assays Enzyme-linked immunosorbent assay Elabscience Cat# E-EL-M0044 TRIzol Invitrogen Cat# 15596018CN HiScript II Q Select RT SuperMix Vazyme Cat# R222-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat# Q511-03 Deposited data 16S rRNA-seq This paper PRJNA1159518, https://www.ncbi.nlm.nih. gov/sra/?term=PRJNA1159518 Proteomics This paper PXD075246, https://proteomecentral. proteomexchange.org RNA-seq This paper PRJNA1345435, https://www.ncbi.nlm. nih.gov/sra/?term=PRJNA1345435 Experimental models: Cell lines RAW264.7 American Type Culture Collection TIB-71 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory IMSR_JAX:000664 Mouse: IL-6 Knockout Xinghua Gao, China Pharmaceutical University N/A Oligonucleotides See Table S1 N/A N/A Software and algorithms GraphPad Prism Graph Pad https://www.graphpad.com/ FlowJo FlowJo https://www.flowjo.com/solutions/flowjo Image-ProPlus software 6.0 Media Cybernetics https://image-pro-plus.software. informer.com/6.0/ Adobe Illustrator Adobe https://www.adobe.com SuperMaze software Shanghai XinRuan Information Technology https://www.shxinruan.com/ Other T. gondii soluble tachyzoite antigen (STAg) This paper N/A 18 Cell Reports 45, 117211, April 28, 2026 .. Offspring from pregnant mice intraperitoneally injected with parasitic mimetics (soluble tachyzoite antigen, STAg) or synthetic double-stranded RNA (dsRNA) Poly(I:C) (Sigma-Aldrich, St. Louis, USA), a viral infection mimic, exhibited behavioral abnormalities, including social deficits, impaired communication, and repetitive behaviors.8,14 For mating, male and female mice were housed together overnight, and females were examined for the presence of seminal plugs the following morning.

    Lysis:

    Article Title: Maternal immune activation perturbs intestinal niche through microbial glycerophospholipids and drives offspring behavioral abnormalities.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER DMEM WISENT Cat# 319-205-CL Tween 20 Sigma-Aldrich Cat# P1379 Triton X-100 Sigma-Aldrich Cat# 93443 bovine serum albumin Sigma-Aldrich Cat# V900933 SDS-PAGE Sample Loading Buffer Beyotime Biotechnology Cat# P0015 RIPA Complete Lysis Buffer Beyotime Biotechnology Cat# P0038 0.25% Trypsin-EDTA (1×) Gibco Cat# 25200072 180 kDa Plus Prestained Protein Marker Vazyme Cat# MP201 DMSO Invitrogen Cat# D12345 InStabTM Protease Cocktail YEASEN Cat# 20124ES03 DAPI Solarbio Cat# 28718-90-3 Critical commercial assays Enzyme-linked immunosorbent assay Elabscience Cat# E-EL-M0044 TRIzol Invitrogen Cat# 15596018CN HiScript II Q Select RT SuperMix Vazyme Cat# R222-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat# Q511-03 Deposited data 16S rRNA-seq This paper PRJNA1159518, https://www.ncbi.nlm.nih. gov/sra/?term=PRJNA1159518 Proteomics This paper PXD075246, https://proteomecentral. proteomexchange.org RNA-seq This paper PRJNA1345435, https://www.ncbi.nlm. nih.gov/sra/?term=PRJNA1345435 Experimental models: Cell lines RAW264.7 American Type Culture Collection TIB-71 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory IMSR_JAX:000664 Mouse: IL-6 Knockout Xinghua Gao, China Pharmaceutical University N/A Oligonucleotides See Table S1 N/A N/A Software and algorithms GraphPad Prism Graph Pad https://www.graphpad.com/ FlowJo FlowJo https://www.flowjo.com/solutions/flowjo Image-ProPlus software 6.0 Media Cybernetics https://image-pro-plus.software. informer.com/6.0/ Adobe Illustrator Adobe https://www.adobe.com SuperMaze software Shanghai XinRuan Information Technology https://www.shxinruan.com/ Other T. gondii soluble tachyzoite antigen (STAg) This paper N/A 18 Cell Reports 45, 117211, April 28, 2026 .. Offspring from pregnant mice intraperitoneally injected with parasitic mimetics (soluble tachyzoite antigen, STAg) or synthetic double-stranded RNA (dsRNA) Poly(I:C) (Sigma-Aldrich, St. Louis, USA), a viral infection mimic, exhibited behavioral abnormalities, including social deficits, impaired communication, and repetitive behaviors.8,14 For mating, male and female mice were housed together overnight, and females were examined for the presence of seminal plugs the following morning.

    Marker:

    Article Title: Maternal immune activation perturbs intestinal niche through microbial glycerophospholipids and drives offspring behavioral abnormalities.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER DMEM WISENT Cat# 319-205-CL Tween 20 Sigma-Aldrich Cat# P1379 Triton X-100 Sigma-Aldrich Cat# 93443 bovine serum albumin Sigma-Aldrich Cat# V900933 SDS-PAGE Sample Loading Buffer Beyotime Biotechnology Cat# P0015 RIPA Complete Lysis Buffer Beyotime Biotechnology Cat# P0038 0.25% Trypsin-EDTA (1×) Gibco Cat# 25200072 180 kDa Plus Prestained Protein Marker Vazyme Cat# MP201 DMSO Invitrogen Cat# D12345 InStabTM Protease Cocktail YEASEN Cat# 20124ES03 DAPI Solarbio Cat# 28718-90-3 Critical commercial assays Enzyme-linked immunosorbent assay Elabscience Cat# E-EL-M0044 TRIzol Invitrogen Cat# 15596018CN HiScript II Q Select RT SuperMix Vazyme Cat# R222-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat# Q511-03 Deposited data 16S rRNA-seq This paper PRJNA1159518, https://www.ncbi.nlm.nih. gov/sra/?term=PRJNA1159518 Proteomics This paper PXD075246, https://proteomecentral. proteomexchange.org RNA-seq This paper PRJNA1345435, https://www.ncbi.nlm. nih.gov/sra/?term=PRJNA1345435 Experimental models: Cell lines RAW264.7 American Type Culture Collection TIB-71 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory IMSR_JAX:000664 Mouse: IL-6 Knockout Xinghua Gao, China Pharmaceutical University N/A Oligonucleotides See Table S1 N/A N/A Software and algorithms GraphPad Prism Graph Pad https://www.graphpad.com/ FlowJo FlowJo https://www.flowjo.com/solutions/flowjo Image-ProPlus software 6.0 Media Cybernetics https://image-pro-plus.software. informer.com/6.0/ Adobe Illustrator Adobe https://www.adobe.com SuperMaze software Shanghai XinRuan Information Technology https://www.shxinruan.com/ Other T. gondii soluble tachyzoite antigen (STAg) This paper N/A 18 Cell Reports 45, 117211, April 28, 2026 .. Offspring from pregnant mice intraperitoneally injected with parasitic mimetics (soluble tachyzoite antigen, STAg) or synthetic double-stranded RNA (dsRNA) Poly(I:C) (Sigma-Aldrich, St. Louis, USA), a viral infection mimic, exhibited behavioral abnormalities, including social deficits, impaired communication, and repetitive behaviors.8,14 For mating, male and female mice were housed together overnight, and females were examined for the presence of seminal plugs the following morning.

    Knock-Out:

    Article Title: Maternal immune activation perturbs intestinal niche through microbial glycerophospholipids and drives offspring behavioral abnormalities.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER DMEM WISENT Cat# 319-205-CL Tween 20 Sigma-Aldrich Cat# P1379 Triton X-100 Sigma-Aldrich Cat# 93443 bovine serum albumin Sigma-Aldrich Cat# V900933 SDS-PAGE Sample Loading Buffer Beyotime Biotechnology Cat# P0015 RIPA Complete Lysis Buffer Beyotime Biotechnology Cat# P0038 0.25% Trypsin-EDTA (1×) Gibco Cat# 25200072 180 kDa Plus Prestained Protein Marker Vazyme Cat# MP201 DMSO Invitrogen Cat# D12345 InStabTM Protease Cocktail YEASEN Cat# 20124ES03 DAPI Solarbio Cat# 28718-90-3 Critical commercial assays Enzyme-linked immunosorbent assay Elabscience Cat# E-EL-M0044 TRIzol Invitrogen Cat# 15596018CN HiScript II Q Select RT SuperMix Vazyme Cat# R222-01 AceQ qPCR SYBR Green Master Mix Vazyme Cat# Q511-03 Deposited data 16S rRNA-seq This paper PRJNA1159518, https://www.ncbi.nlm.nih. gov/sra/?term=PRJNA1159518 Proteomics This paper PXD075246, https://proteomecentral. proteomexchange.org RNA-seq This paper PRJNA1345435, https://www.ncbi.nlm. nih.gov/sra/?term=PRJNA1345435 Experimental models: Cell lines RAW264.7 American Type Culture Collection TIB-71 Experimental models: Organisms/strains Mouse: C57BL/6J The Jackson Laboratory IMSR_JAX:000664 Mouse: IL-6 Knockout Xinghua Gao, China Pharmaceutical University N/A Oligonucleotides See Table S1 N/A N/A Software and algorithms GraphPad Prism Graph Pad https://www.graphpad.com/ FlowJo FlowJo https://www.flowjo.com/solutions/flowjo Image-ProPlus software 6.0 Media Cybernetics https://image-pro-plus.software. informer.com/6.0/ Adobe Illustrator Adobe https://www.adobe.com SuperMaze software Shanghai XinRuan Information Technology https://www.shxinruan.com/ Other T. gondii soluble tachyzoite antigen (STAg) This paper N/A 18 Cell Reports 45, 117211, April 28, 2026 .. Offspring from pregnant mice intraperitoneally injected with parasitic mimetics (soluble tachyzoite antigen, STAg) or synthetic double-stranded RNA (dsRNA) Poly(I:C) (Sigma-Aldrich, St. Louis, USA), a viral infection mimic, exhibited behavioral abnormalities, including social deficits, impaired communication, and repetitive behaviors.8,14 For mating, male and female mice were housed together overnight, and females were examined for the presence of seminal plugs the following morning.



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